Gel Electrophoresis Buffers and Diluents
ILEX LIFE SCIENCES LLC 1X RBC LYSIS BUFFER 100 ML
PAN-Biotech 1x Erythrocyte Lysis Buffer (1x RBC Lysis Buffer), 100 ml. DESCRIPTION: In normal whole-blood, erythrocyte concentration is 1000x higher than leukocyte concentration, making erythrocytes an impediment to investigation or analysis of leucocytes. Erythrolyse, or red blood cell lysing buffer is intended for the lysis of red blood cells in samples with white blood cells as for example EDTA-/citrate/heparin-treated whole-blood, Buffy Coats (PBMCs, leucocyte films), and bone marrow. This buffer contains ammonium chloride, which lyses red cells, with minimal effect on lymphocytes when used as instructed. Nucleated red cells are not effectively lysed with ammonium chloride. COMPOSITION: Contains ammonium chloride, potassium hydrogencarbonate, and EDTA. Erythrocyte Lysis Buffer (1x) is sterile filtered (0.2 μm) and contains no fixative and no preservatives. CONCENTRATION: 1X. LIQUID/POWDER: Liquid. STERILE: Yes. STORAGE TEMP: +2°C - RT. MANUFACTURER: PAN-Biotech GmbH (Germany).
Bioworld Laemmli SDS-Sample Buffer 6X, Reducing 25ml
An electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Technical Information: Ultra-Pure Grade, Contents of a 1X Solution: 1.5% SDS and 10% glycerol, 62.5mM Tris -HCl (pH 6.8), 0.0025% Bromophenol blue, 2% β-mercaptoethanol. DNase: None detected, RNase: None detected, Proteases: None detected, Stability (-20°C) >1 Year. Appearance Form : Liquid, Appearance Color : Purple. 25ml
Bioworld Alkaline DNA Gel Running Buffer, 500 mL
Product DescriptionAlkaline DNA gel running buffer contains 0.5M NaOH and 50 mM EDTA. EDTA is to chelate divalent metal cations such as Mg2+ and Ca2+, which are required for DNA function of degrading enzymes (DNAses).
Bioworld Laemmli SDS-Sample Buffer (4X), Reducing, 25 mL
Laemmli (SDS-Sample) 4X Buffer, Reducing An electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Technical Information:Ultra Pure GradeContents of a 1X Solution: 1.5% SDS and 10% glycerol, 62.5mM Tris -HCl (pH6.8), 0.0025% Bromophenol blue, 2% β-mercaptoethanol.DNase: None detectedRNase: None detectedProteases: None detected
Bioworld Laemmli SDS-Sample Buffer (6X), Non-Reducing, 25 mL
Laemmli (SDS-Sample) 6X Buffer, Non-Reduced An electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Composition of this buffer is similar to the reducing buffer minus mercaptoethanol. Technical Information:Ultra Pure GradeContents of a 1X Solution: 2% SDS and 10% glycerol, 62.5mM Tris -HCl (pH 6.8), 0.0025% Bromophenol blue.DNase: None detectedRNase: None detectedProteases: None detectedStability (4°C): for 1year
Bioworld Duplex Buffer, 100 mL
Product Description Duplex buffer contains 100 mM potassium acetate, 30 mM HEPES and has a pH level of 7.5. It comes as a sterile solution in DEPC-treated water and is for initial resuspension, annealing and storage of duplex DsiRNA products. The salt is necessary for oligonucleotide hybridization.
Boston Bioproducts Inc TE Buffer pH 8 250ml
Tris-EDTA Buffer TE (pH 8 0) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc EBC Lysis Buffer - 500ml
EBC Lysis Buffer - 500ml (For Research Use and Further Manufacturing Only)
Cell Signaling Technology FoxP3/Transcription Factor Permeabilization Buffer (10X) 150 ml
FoxP3/Transcription Factor Permeabilization Buffer (10X) 150 ml
Research Products International Corp EKONO Hybridization Buffer, 1 Liter
EKONO provides real cost savings without sacrificing performance or reliability. Effective for Southern, Northern, Southwestern and oligonucleotide hybridizaiton. Can be used with radioactive and non-radioactive probes or for screening libraires. Simple overnight incubation produces high sensitivity with minimum background.
Supplied in a 1 liter bottle.
Store at room temperature.
Vector Laboratories BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit 200 ml
BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit, 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium, forms an indigo, heat-stable reaction product that can be dehydrated, cleared, and permanently mounted. This substrate can also be used for developing blots or for in situ hybridization. With 20-30 minute development times, the sensitivity of this substrate is equivalent to the other alkaline phosphatase substrates. However, BCIP/NBT will continue to develop from several hours to overnight making it one of the most sensitive chromogenic substrates. The stock solutions in this kit are provided in convenient dropper bottles, in sufficient volume for 200 ml working solution.